expression plasmid pet30a Search Results


99
New England Biolabs pet30a expression vector
Pet30a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation pet30a(+) expression plasmid
Pet30a(+) Expression Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation custom synthesized segment of the porcine cd163 receptor gene
Custom Synthesized Segment Of The Porcine Cd163 Receptor Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TransGen biotech co pet30a-aahd1
Pet30a Aahd1, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc full length anp32a pet30a pp32
Full Length Anp32a Pet30a Pp32, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA pet30a expression vector
Pet30a Expression Vector, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc expression vector
Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Solarbio Inc pet-30a(+) expression vector
Pet 30a(+) Expression Vector, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs expression vector pet30a
Expression Vector Pet30a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega pet30a prokaryotic expression vector
Pet30a Prokaryotic Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pet30a
Amplification and cloning of chitinase I gene in TA vector and in E. coli expression vector <t>pET30a.</t> (A) Lane 1: 1 kb DNA ladder; lanes 2–4: samples showing amplification of ∼935 bp of chitinase I gene. (B) Restriction digestion of plasmid pTA:chiI harbouring chitinase I gene cloned in pCR2.1 vector with EcoRI enzyme. Lanes 1–2: pTA:chiI DNA samples; M represent 1 kb DNA ladder. The release of ∼3.9 kb DNA fragment represent pCR2.1 vector while ∼935 bp depict chitinase gene. (C) Restriction digestion of pET-chiI recombinant plasmid with EcoRI to confirm ligation of chitinase gene in E. coli expression vector. Lane M: 1 kb DNA ladder, lanes 1–3: pET-chiI plasmid DNA. The release of ∼5.4 kb DNA fragment represent pET30a vector while ∼935 bp depict chitinase gene.
Pet30a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation gene fragments
Amplification and cloning of chitinase I gene in TA vector and in E. coli expression vector <t>pET30a.</t> (A) Lane 1: 1 kb DNA ladder; lanes 2–4: samples showing amplification of ∼935 bp of chitinase I gene. (B) Restriction digestion of plasmid pTA:chiI harbouring chitinase I gene cloned in pCR2.1 vector with EcoRI enzyme. Lanes 1–2: pTA:chiI DNA samples; M represent 1 kb DNA ladder. The release of ∼3.9 kb DNA fragment represent pCR2.1 vector while ∼935 bp depict chitinase gene. (C) Restriction digestion of pET-chiI recombinant plasmid with EcoRI to confirm ligation of chitinase gene in E. coli expression vector. Lane M: 1 kb DNA ladder, lanes 1–3: pET-chiI plasmid DNA. The release of ∼5.4 kb DNA fragment represent pET30a vector while ∼935 bp depict chitinase gene.
Gene Fragments, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Amplification and cloning of chitinase I gene in TA vector and in E. coli expression vector pET30a. (A) Lane 1: 1 kb DNA ladder; lanes 2–4: samples showing amplification of ∼935 bp of chitinase I gene. (B) Restriction digestion of plasmid pTA:chiI harbouring chitinase I gene cloned in pCR2.1 vector with EcoRI enzyme. Lanes 1–2: pTA:chiI DNA samples; M represent 1 kb DNA ladder. The release of ∼3.9 kb DNA fragment represent pCR2.1 vector while ∼935 bp depict chitinase gene. (C) Restriction digestion of pET-chiI recombinant plasmid with EcoRI to confirm ligation of chitinase gene in E. coli expression vector. Lane M: 1 kb DNA ladder, lanes 1–3: pET-chiI plasmid DNA. The release of ∼5.4 kb DNA fragment represent pET30a vector while ∼935 bp depict chitinase gene.

Journal: Brazilian Journal of Microbiology

Article Title: Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host

doi: 10.1016/j.bjm.2017.05.007

Figure Lengend Snippet: Amplification and cloning of chitinase I gene in TA vector and in E. coli expression vector pET30a. (A) Lane 1: 1 kb DNA ladder; lanes 2–4: samples showing amplification of ∼935 bp of chitinase I gene. (B) Restriction digestion of plasmid pTA:chiI harbouring chitinase I gene cloned in pCR2.1 vector with EcoRI enzyme. Lanes 1–2: pTA:chiI DNA samples; M represent 1 kb DNA ladder. The release of ∼3.9 kb DNA fragment represent pCR2.1 vector while ∼935 bp depict chitinase gene. (C) Restriction digestion of pET-chiI recombinant plasmid with EcoRI to confirm ligation of chitinase gene in E. coli expression vector. Lane M: 1 kb DNA ladder, lanes 1–3: pET-chiI plasmid DNA. The release of ∼5.4 kb DNA fragment represent pET30a vector while ∼935 bp depict chitinase gene.

Article Snippet: A ∼935 bp chitinase I gene was ligated in pET30a (Addgene) expression vector having N/C-terminal His-tag sequence and expressed under T7 promoter to create a pET-chiI construct.

Techniques: Amplification, Cloning, Plasmid Preparation, Expressing, Clone Assay, Recombinant, Ligation