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Image Search Results
Journal: Brazilian Journal of Microbiology
Article Title: Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
doi: 10.1016/j.bjm.2017.05.007
Figure Lengend Snippet: Amplification and cloning of chitinase I gene in TA vector and in E. coli expression vector pET30a. (A) Lane 1: 1 kb DNA ladder; lanes 2–4: samples showing amplification of ∼935 bp of chitinase I gene. (B) Restriction digestion of plasmid pTA:chiI harbouring chitinase I gene cloned in pCR2.1 vector with EcoRI enzyme. Lanes 1–2: pTA:chiI DNA samples; M represent 1 kb DNA ladder. The release of ∼3.9 kb DNA fragment represent pCR2.1 vector while ∼935 bp depict chitinase gene. (C) Restriction digestion of pET-chiI recombinant plasmid with EcoRI to confirm ligation of chitinase gene in E. coli expression vector. Lane M: 1 kb DNA ladder, lanes 1–3: pET-chiI plasmid DNA. The release of ∼5.4 kb DNA fragment represent pET30a vector while ∼935 bp depict chitinase gene.
Article Snippet: A ∼935 bp chitinase I gene was ligated in
Techniques: Amplification, Cloning, Plasmid Preparation, Expressing, Clone Assay, Recombinant, Ligation